(a) PCL sponge 26 w/v%, 15 cm, 20 kV, and 10,000 µL/h, 26 °C, 60% humidity, SEM image showing the roughness of the fibers. (b) SEM showing fiber 80:20 PCL/gel 24 w/v% on foil, 24 °C, 31% RH, 1500 µL/h, 15 cm, 22 kV webbing and (c) 80:20 PCL/gel sponge at 24 w/v%, 78% RH, 30 °C, 1500 µL/h, 15 cm, 22 kV shows loss of webbing and fiber porosity. Images were taken with a field view of 15 µm. Credit: Nanomaterials (2023). DOI: 10.3390/nano13243107

A new technique for electrospinning sponges has allowed scientists at the University of Surrey to produce direct 3D scaffolds – onto which skin grafts can be grown from a patient’s own skin.

Electrospinning is a technique that electrifies liquid droplets to create fiber from plastic. Earlier, scientists could only make 2D movies. This is the first time anyone has electrospun a 3D structure directly and on-demand to produce it at scale. Here is the research. published In the journal Nanomaterials.

“After spinning these scaffolds, we grew skin cells on them,” said Chloe Howard, from Surrey’s School of Computer Science and Electronic Engineering. After seven days, they were twice as large as cells grown on 2D films or mats. were viable. Compared to cells grown on plasma-treated polystyrene—first, the gold standard. They were very happy cells on our 3D scaffolds.

“Our findings pave the way for the patient’s own harvesting. And multiply them. These grafts can heal chronic wounds better and faster.”

The scientists developed a solution that included gelatin and polycaprolactone (PCL) – a biodegradable polymer that is compatible with it. . They pumped the solution through a syringe into an electric field, which caused it to expand into nanofibers.

The process is simple, scalable and inexpensive. Researchers now hope it can be used in others .

“Electro-spinning is highly applicable. We can mimic the way muscle fibers behave by spinning fibers that run in the same direction,” said Dr Vlad Stoljan, Associate Professor at Surrey’s Institute of Advanced Technology. “This technique could one day create artificial skin, bone. And cartilage, too — helping people recover from injuries faster, and with better long-term outcomes.”

More information:
Chloe Jayne Howard et al, Preparation conditions for direct and reproducible fabrication of electrospun PCL/gelatin 3D structures for tissue regeneration, Nanomaterials (2023). DOI: 10.3390/nano13243107

Reference: Lab-spun sponges form excellent scaffolds for skin cell growth to heal wounds (2024, February 23) https://phys.org/news/2024-02-lab-spun February 23, 2024 Retrieved from -sponges-scaffolds-skin. html

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